Introduction: angiogenesis is the process of blood vessel formation from a preexisting vascular network, which is augmented in pathological processes such as cancer. Medulloblastoma (MB) and primitive neuroectodermal tumor (PNET), are both embryonic tumors of the central nervous system of high mitotic rate and preferential dissemination via cerebrospinal fluid (CSF). Betamethasone (β-met) is a glucocorticoid with antiangiogenic effect. Material and method: We used the alantochorionic membranes assay (CAM) to measure angiogenesis with 10 μL of CSF from patients with MB and PNET, realizing a comparative study of the angiogenic effect of of β-met at a concentration of 0.08μg/mL in situ, CSF of patients which received β-met intravenous and untreated with the drug. The control group corresponds to a sample of normal CSF. Microvascular density in an area of 9000μm2 was determined. Results: The statistical study (ANOVA) showed significant differences (p < 0.01) in the microvascular density compared to experimental groups. The blood vessels average in the different groups were 15, 4 vessels/area (control), 10,2 vessels/area (β-met iv), 17,3 vessels/area (without β-met) and 13 vessels/area (β-met in situ). Discussion: These results suggest a possible antiangiogenic effect of betamethasone in doses used intravenously.
Palabras clave:
Meduloblastoma, Betametasona, Inductores de la Angiogénesis
Valdés A., C. ., Valenzuela A., A., Sinning, M. ., Rosas C., C. ., & Lemus A., D. (2013). Efecto de la betametasona sobre la actividad angiogénica en líquido cerebroespinal (lCE) en pacientes adultos portadores de meduloblastomas/PNET. Revista Hospital Clínico Universidad De Chile, 24(2), pp. 105–11. https://doi.org/10.5354/2735-7996.2013.73330
